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Measurement of the distribution of an equivalent of cell dry mass using quantitative phase contrast of the CCHM
Kovářová, Klára ; Čolláková, Jana (referee) ; Křížová, Aneta (advisor)
Subject of this bachelor's thesis is to create an overview of conversions of phase profile to the values of dry mass and to realize experiments verifying the importance of dry mass. In the theoretical part, the history of the holographic microscopy and the development of the holographic microscopy on IPE FME BUT in Brno are summarized. The transmission holographic microscope is also described. This thesis covers its construction description and basic working instructions. The theoretical part also deals with the importance of image phase. Two approaches are presented for the interpretation of the phase delay and there is research on phase conversions to dry cell mass (according to H. G. Davies and M. H. F. Wilkins, G. Popescue, G. Dunn and D. Zicha). In the experimental part cultivation of cells, preparation of observation sample and processing of data are described. This part of thesis is also focused on laboratory experiments. Cells K2 (full name LW13K2) were observed in all experiments. First experiments deal with nutritional deprivation of cells and later with cell growth.
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Perception of the Cell wall integrity system signals in plant cells
Hercíková, Anna ; Fendrych, Matyáš (advisor) ; Cifrová, Petra (referee)
The cell wall is a key compartment of the plant cell for elementary physiological processes such as cell growth, division, or differentiation, simultaneously protecting the cell from influences of the external environment. The mechanical support of the cell wall maintains the shape of the cell, and at the same time allows it to grow. Thus, the cell wall must not be limiting to cell expansion, but if it becomes too loose, the cell may rupture. Supervised adaptive reorganisation of the cell wall based on external and internal conditions is therefore essential for plant cell, as indicated by the presence of a complex signalling system. The Cell wall integrity (CWI) system represents the set of all mechanisms that together ensure the continuous compactness of the cell wall. My bachelor thesis will discuss the individual components of the CWI system, focusing on the CrRLK1Ls, receptors from the broader Receptor-like kinase (RLK) family.
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Molecular basis for regulation of cell wall pH in Arabidopsis thaliana
Bogdan, Michal ; Fendrych, Matyáš (advisor) ; Retzer, Katarzyna (referee)
The cell wall is one of the defining parts of the plant cell. Cell walls resist turgor pressure, give plants their rigidity and still allow the cell to grow despite their stiffness. The plant cell wall is a composite material that consists of different elements; thus, the knowledge of the plant cell wall composition helps us understand the impact of the mentioned pH changes. Alterations of plant cell wall properties result in a change of plant cell growth rate. A prime example of this is the modification of wall properties by pH change, termed the acid growth. Although acid growth has been studied for a long time, we still do not fully understand the underlying mechanisms, as we have not yet identified all the agents involved in acid growth and our ability to determine apoplastic pH is limited. Local cell wall pH fluctuations arise also due to plant organs having different roles and being affected by a diverse range of stimuli. Despite the fact that new pH measurement techniques, such as genetically encoded fluorescent probes, were developed in the last two decades, there is still a need to provide a higher spatiotemporal resolution of pH analysis. Key words: Arabidopsis, apoplastic pH, cell growth, acid growth theory, cell wall, auxin, apoplastic pH measurement
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Measurement of the distribution of an equivalent of cell dry mass using quantitative phase contrast of the CCHM
Kovářová, Klára ; Čolláková, Jana (referee) ; Křížová, Aneta (advisor)
Subject of this bachelor's thesis is to create an overview of conversions of phase profile to the values of dry mass and to realize experiments verifying the importance of dry mass. In the theoretical part, the history of the holographic microscopy and the development of the holographic microscopy on IPE FME BUT in Brno are summarized. The transmission holographic microscope is also described. This thesis covers its construction description and basic working instructions. The theoretical part also deals with the importance of image phase. Two approaches are presented for the interpretation of the phase delay and there is research on phase conversions to dry cell mass (according to H. G. Davies and M. H. F. Wilkins, G. Popescue, G. Dunn and D. Zicha). In the experimental part cultivation of cells, preparation of observation sample and processing of data are described. This part of thesis is also focused on laboratory experiments. Cells K2 (full name LW13K2) were observed in all experiments. First experiments deal with nutritional deprivation of cells and later with cell growth.
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